Biochemist - Category Number 232/2024
Sample Questions Question 1 Q: Which type of chromatography is most suitable for separating small polar biomolecules such as amino acids and sugars? Answer: Ion-exchange chromatography (particularly anion or cation exchange depending on molecule charge) or HPLC with appropriate polar stationary phase; for many small polar molecules, ion-exchange is commonly used. Question 2 Q: Describe the Michaelis–Menten constant (Km) and its significance in enzyme kinetics. Answer: Km is the substrate concentration at which the reaction rate is half of Vmax. It reflects the affinity of the enzyme for its substrate (lower Km = higher affinity) under Michaelis–Menten conditions. Question 3 Q: What is the primary clinical significance of elevated serum alanine aminotransferase (ALT)? Answer: Elevated ALT is primarily a marker of hepatocellular injury or liver cell damage and is commonly used to assess liver disease or acute hepatic injury. Question 4 Q: Name two quality cont...